STRENGTHENING OF PRACTICAL SKILLS


Dept Practical / Demonstration UG Batch
Biotec a. Preparation of Mol Bio Stock & Working solutionsb. Isolation & characterization of DNA from different sources B.Sc.BTIII
c. Separation of PBMCs B.Sc.BTII
d. Diagnostic ELISA assay;e. DID assay BT I Sem; II;III Y
f. Biochemical tests useful in bacterial taxonomyg. Estimation of amylase activity

h. Effect of temperature &pH on growth of given microbe BT I Sem; II Y
B.Sc.BTII
Bot i. Micro propagation;j. Rooting & Shooting of explants;

k. Acclimatization of plantlets

l. Potato osmometer m. moisture content of leaves

m. rate of respiration of various plant parts

B.Sc. BTIII; Med III
Che n. Preparation of Benzilic acid from benzil by micro oveno. Spectral data analysis of aspirin, Caffeine, Salicyclic acid, Methyl orange, para nitro acetanilide B.Sc. BTIII; B.Sc. Med III
Phy p. Spectrometric analysisq. Angle of rotation by polarimeter B.Sc. BT II, III; B.Sc. Med III
Zoo r. Diagnostic ELISA assay; s. DID assay B.Sc. BTII; Med III
Biotec a. Preparation of Mol Bio Stock & Working solutions; b. Isolation &characterization of DNA from human blood. c. Qualitative & quantitative analysis of DNA; d. AFB microscopy; e. Culturing of M. tuberculosis; f. M.tb DNA isolation; g. PCR assays; h. AGE; i. RE digestion B.Sc.BTIII
j. Diagnostic ELISA assay; k. DID assay; l. Haemagglutination assay; m.Separation of PBMCs & viability test; n. Plate adhesion assay; o. Reverse

blood group typing; p. Absorption maxima of mixture of 2 dyes; q.

Biochemical tests useful in bacterial taxonomy; r. Estimation of glucose;

s. Estimation of amylase activity

B.Sc.BTIII Sem
t. Effect of temperature &pH on growth of given microbe B.Sc.BTI Sem
u. RID; v. Antibiotic sensitivity; w. MIC B.Sc. BT IV sem
x. Water quality testing B.Sc.BT II Sem
Bot a.Micro propagation; b. Rooting & Shooting of explants; c. Acclimatization of plantlets d. Seed culture B.Sc. BTIII; Med III; B..Sc. Med Sem V
e. Floral study (Luffa, Rose, Sunflower, Ageratum, Sonchus, Poppy); f. Study plant movements B.Sc. Med III Sem
g. Making Herbarium; h. Project on available flowering plants in campus and their mode of pollination B. Sc. Med IV sem
Che a. Preparation of Benzilic acid from benziyl; b. Spectral data analysis of aspirin,Caffeine, Salicyclic acid, Methyl orange, para nitro acetanilide B.Sc. BTIII; B.Sc. Med III
c. Chromatographic separation of leaf pigments and dyes; d. Caffeine extraction; e.Photoreduction of Benzoquinone B.Sc. (Med/ NM/ BT) III
Phy a. Demo of GM counter; b. Angle of rotation by polarimeter B.Sc. BT II, IV sems
c. Demo of Scintillation counter; d. Demo of Hydrogen spectrum B.Sc. NM III; IV sem
e. Demo of the change in magnetization intensity for different magnetic materials and to trace the area under the B-H curve B.Sc. NM III
Zoo a. Diagnostic ELISA assay B.Sc. Med IV sem
b. DNA extraction B.Sc. Med I sem
c. AGE B.Sc IV sem BT voc
Biotec a. PCR assay b. Validation of PCR assay by RE digestion c. Elution of DNA from Agarose gel d. Primer designing; e. Extraction of human genomic DNA form peripheral blood employing organic vs inorganic method; f. Protein extraction & quantification from pulses B.Sc.BTVI Sem
g. RID; h. Comparative performance of plant DNA extraction protocols employing ammonium acetate vs CI as deproteinization agents; i. Acid phosphatase isolation & quantification from Mungbean; j. Isolation of starch hydrolysing amylase producing bacterial cultures; k. Antibiotic senstivity; l. MIC B.Sc.BTIV Sem
m. Water quality testing B.Sc.BT II Sem
n. Reverse blood group typing; o. Staining of blood smear by Giemsa stain; p. Isolation of WBCs; q. Plate adhesion assay B.Sc. BT Sem III
Bot a. Meristem culture B.Sc. BTIII; Med III; B..Sc. Med Sem V
b. Floral study (Petunia & Rice) B.Sc. Med III Sem
c. collection of variety of dicot and monocot leaves from college campus and study the leaf characteristics B. Sc. Med IV sem
d. Floral study of Rose,Nerium, Thevetia B.Sc. BT Sem I
e. collection of economically important dried parts of plants. B.Sc. Med Sem VI
Phy To demonstrate the effect of adiabatic expansion and compression of gas in system. B.Sc. NM II
To realize practical the value of “stopping potential” for the different coloured lights by using filters which is helpful for the calculation of Plank’s constant.
To study the “optical activity” of the solution and its dependence on different concentrations of solution. B.Sc. NM IV
To demonstrate that how magnetization intensity is going to change with applied magnetic field for soft and hard magnetic materials.
To demonstrate Scintillation counter and explain how photomultiplier tube (PMT) works. B.Sc. NM VI
Use of spectrometer to study the light from the object into a spectrum, a rainbow of colours by placing prism or diffraction grating in front of source.